It requires as little as 20μL of neat sample and as few as 1,000-2,000 total cells to perform an analysis and works with both adherent and non-adherent cells.
The system uses a patented flow cell that is made of fused silica with a polyimide coating that requires no alignment following replacement; there is a high-pressure purge of the flow cell available for removing obstructions in the flow cell and the sample is aspirated through the flow cell using a high pecision microsyringe.
The system requires minimal maintenance with no laser alignment and a user replaceable flow cell and generates minimal waste of <80mL in a 8 hours of running sample.
The system uses a modular software package called CytoSoft that allows you the option of choosing from any of the following assays: Cell counts/viabilities, Apoptosis (Annexn-V binding, pan caspases, TUNEL), cell cycle, cytotoxicity, cell painting, and other multi-color detection assays (up to three colors).
The system has a very small footprint (~1.0’ H x 1.5’ W x 2.0’ D), requiring minimal space on the benchtop.
The Guava easyCyte 8HT System provides absolute cell counts and offers other high-demand features, such as eight parameters (6 fluorescent colors, 2 light scatter) and has a blue laser and red laser for access to commonly used fluorescent dyes.
It also offers a built-in 96-well autosampler, which allows researchers to obtain even more data while performing more complex biological studies such as white blood cell phenotyping, cell signaling, cytokine production, activation marker analysis, and multiplex compound screening. Monitoring the interplay of up to eight different biological mechanisms simultaneously improves the efficiency of experimental outputs. Not only does this shorten the number of iterations for any one experiment, it also comes that much closer to mimicking the complex biological responses within the body.
The Guava easyCyte 8HT System runs all Guava Assays and uses Guava InCyte software for data acquisition and analysis. Guava InCyte offers drag and drop gating, pre and post acquisition compensation, heat map display and IC50/EC50 curve generation.
- NIR2: 785/70 nm
- Red2: 661/19 nm
- NIR1: 785/70 nm
- Red1: 692/46 nm
- Yellow: 583/26 nm
- Green: 525/.30 nm
- 96-well plates
- 1.2 or 1.5 mL tubes
10 - 500 cells/uL
- Stem Cell Research
- QC & Maintenance
- Chemokine Analysis
- Cell Tracking
- Cell Cycle
- Cell Counting & Viability
- Apoptosis & Cancer
- Antibody Quantitation
12 uL/min to 60 uL/min
- Blue Laser: 488 nm
- Red Laser: 640 nm
- Option: Integrate with external robotic arm or liquid handler via Guava Link Software
- Laptop computer with optional mouse
- Standard built-in 96-well plate/10 tube format
- Automated analyzer
10” (25.4 cm) h x 20.3” (51.5 cm) w x 23.4” (59 cm) d