3.6
5 reviews
71

Milipore Guava easyCyte 8/8HT


Released January, 2010

Product Shot 1 The Pros:Another benefit of this machine is that is does not use shealth fluid, which saves on the amount of biohazard waste that you have to deal with. I disagree with the comment about clogging. I have had an easycyte instrument for a number of years and when your experiment is setup correctly, you will not have clogging issues. Mechanically simple. Easy maintenance. I have not had clogging except when my samples were too concentrated. Back-flush (built-in option) always declogged the flow-cell. Usable analysis software.

The Cons:Limited to 2 excitation lasers (488 and 640 nm). Need to switch tubes around between cleaning and analyzing cycles. Software is decidedly non-user-friendly. As is documentation. Clogging problems are a pain. There is a distinct limitation in using this system where there is autofluorescence or unbound dye.

The Guava EasyCyte system is the only benchtop flow microcapillary system that allows you the option of performing single-cell analysis for multiple assays in a 96-well format. It accommodates both 96-well microplates and 0.5 mL and 1.5 mL microcentrifuge tubes.

It requires as little as 20μL of neat sample and as few as 1,000-2,000 total cells to perform an analysis and works with both adherent and non-adherent cells. 

The system uses a patented flow cell that is made of fused silica with a polyimide coating that requires no alignment following replacement; there is a high-pressure purge of the flow cell available for removing obstructions in the flow cell and the sample is aspirated through the flow cell using a high pecision microsyringe

The system requires minimal maintenance with no laser alignment and a user replaceable flow cell and generates minimal waste of <80mL in a 8 hours of running sample. 

The system uses a modular software package called CytoSoft that allows you the option of choosing from any of the following assays: Cell counts/viabilities, Apoptosis (Annexn-V binding, pan caspases, TUNEL), cell cycle, cytotoxicity, cell painting, and other multi-color detection assays (up to three colors). 

The system has a very small footprint (~1.0’ H x 1.5’ W x 2.0’ D), requiring minimal space on the benchtop. 



The Guava easyCyte 8HT System provides absolute cell counts and offers other high-demand features, such as eight parameters (6 fluorescent colors, 2 light scatter) and has a blue laser and red laser for access to commonly used fluorescent dyes. 
It also offers a built-in 96-well autosampler, which allows researchers to obtain even more data while performing more complex biological studies such as white blood cell phenotyping, cell signaling, cytokine production, activation marker analysis, and multiplex compound screening. Monitoring the interplay of up to eight different biological mechanisms simultaneously improves the efficiency of experimental outputs. Not only does this shorten the number of iterations for any one experiment, it also comes that much closer to mimicking the complex biological responses within the body. 
The Guava easyCyte 8HT System runs all Guava Assays and uses Guava InCyte software for data acquisition and analysis. Guava InCyte offers drag and drop gating, pre and post acquisition compensation, heat map display and IC50/EC50 curve generation.

 
Emission:
  • NIR2: 785/70 nm
  • Red2: 661/19 nm
  • NIR1: 785/70 nm
  • Red1: 692/46 nm
  • Yellow: 583/26 nm
  • Green: 525/.30 nm
 
Scatter:
  • Side
  • Forward
 
Sample Format:
  • 96-well plates
  • 1.2 or 1.5 mL tubes
 
Sample Volume, µl:
125
 
Cell Number per Assay:
10 - 500 cells/uL
 
Flow Cytometry Research Category:
  • Stem Cell Research
  • Signaling
  • QC & Maintenance
  • Chemokine Analysis
  • Cell Tracking
  • Cell Cycle
  • Cell Counting & Viability
  • Apoptosis & Cancer
  • Antibody Quantitation
 
Throughput:
12 uL/min to 60 uL/min
 
Excitation:
  • Blue Laser: 488 nm
  • Red Laser: 640 nm
 
Components:
  • Option: Integrate with external robotic arm or liquid handler via Guava Link Software
  • Laptop computer with optional mouse
  • Standard built-in 96-well plate/10 tube format
  • Automated analyzer
 
Dimensions:
10” (25.4 cm) h x 20.3” (51.5 cm) w x 23.4” (59 cm) d

User Reviews (5)

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Add Pros & Cons
71
ProScore
Pros
  • 2

    Another benefit of this machine is that is does not use shealth fluid, which saves on the amount of biohazard waste that you have to deal with.

  • 1

    I disagree with the comment about clogging. I have had an easycyte instrument for a number of years and when your experiment is setup correctly, you will not have clogging issues.

  • 1

    Mechanically simple. Easy maintenance. I have not had clogging except when my samples were too concentrated. Back-flush (built-in option) always declogged the flow-cell. Usable analysis software.

Cons
  • 1

    Limited to 2 excitation lasers (488 and 640 nm). Need to switch tubes around between cleaning and analyzing cycles.

  • 0

    Software is decidedly non-user-friendly. As is documentation. Clogging problems are a pain. There is a distinct limitation in using this system where there is autofluorescence or unbound dye.

Comments (2)

What's on your mind? See more ProductWiki Talk
thomas Sheehy
thomas Sheehy: #milipore_guava_easycyte_8_8ht I have an easycyte 8ht, changing the tubes is a pain between analysis and cleaning that is the only minor pain I can agree with. I just small particles around 0.5um and the system sees them better than my Canto. Also it can move through a plate faster than I ever expected. We have also done carry over studies and have seen hardly anything. We go through a ton of plates per day and if this was a sheath based system we would be screwed. I head there is a 3rd laser ( 405nm) available now. This is a 10 color instrument. All and all We are happy with the system. You get a lot for the money and all the analysis we have done can be cross validated on our BD canto....which we hardly use anymore because we dont have the HT attachment. Feb 13, 14
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Dr M J Bordoloi
comments (1)
  • thomas Sheehy

    thomas Sheehy: 2 laser / 6 color with software and training we paid $83,000 Feb 13, 14

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