iCyt Synergy

Released January, 2010

Product Shot 1


The Synergy system can be configured as a single channel

cell sorter or analyzer, dual channel sorter or analyzer, or a

sorter/analyzer combination.

Standard bench-top model dimensions:

48" w x 26" d x 30" h

Bio-Safety (BSC) model dimensions:

53" w x 48" d x 89" h



Sorting modules can be configured for either jet-in-air or

gel-coupled sense-in-channel operation.

Product Shot 2

Sorting nozzle sizes include 70 μm, 85 μm, 100 μm, 130 μm

Accommodates particles up to 40μm

Provides 4-way sorting into most popular collection tube

formats. Optional Sort Deposition System (SDS) supports

sorting into most popular plate formats.

Analyzer modules are configured for closed-loop gelcoupled

sense-in-channel operation. Automated sample

delivery system supports most popular plate and tube



Each sorter or analyzer module can be configured with up

to eight lasers focused on five spatially separated

interrogation points.

Standard laser options

Standard laser options include Omicron LuxX™ 375nm,

405nm, 445nm, 488nm, 642nm diode lasers and Cobalt

Calypso™ 491nm, Samba™ 532nm, Jive™ 561nm,

Mambo™ 594nm DPSS lasers.

Standard beam size is approximately 15 μm x 60 μm.

Beam spots are spatially separated by more than 350 μm

to eliminate laser crosstalk.


A multi-interrogation point Reflective Collection Optic

(RCO) provides exceptional fluorescence sensitivity,

resolution, and spatial isolation with an effective NA of 1.2

at five beam spots. Fiber optic cables deliver light to userconfigurable

PMT arrays.

Each sorter or analyzer module can be configured with up

to 32 detectors: up to 26 fluorescence PMTs and 6 scatter


PMT arrays can be configured to detect all popular

fluorochromes and fluorescent proteins including:

375nm - DAPI, Hoechst Blue/Red

405/445nm - CFP, BFP, Pacific Bue, Pacific Orange, QDots,

488nm - GFP, YFP, FITC, PE, PE-TR, PI, PerCP-Cy5.5, PE-Cy5.5,


532/561nm - RFP, dsRed, mCherry, PE, PE-TR, PI, PE-Cy5,

PE-Cy5.5, PE-Cy7

594/642nm - Alexa Fluor® 594, APC, Alexa Fluor® 700,

APC-Alexa Fluor® 750


Each SynergyTM sorter and analyzer module can acquire

up to 24 simultaneous parameters with 105 MHz sampling

at up to 100,000 events per second.

Linear area, linear height, linear width, log area, log

height, and log width pulse features are available.

Full color- compensation matrix via WinList acquisition and

analysis software.

Sense-in-channel sorting at up to 35,000 events per


Jet-in-air sorting at up to 70,000 events per second.


Forward Scatter resolves 0.5μm diameter particles from


Fluorescent Sensitivity - Measured using Sphero™ Rainbow

Particles RCP-30-5A:


PE < 100 MESF

Sort Performance

Purity > 99%

Yield > 90% of theoretical based on Poisson statistics

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