2.2
3 reviews
45

iCyt Reflection


Released January, 2007

Product Shot 1 The Pros:Super fast sorting at 99% accuracy. Very reliable, easy to use software. Versatile, with 4 way sorting on each HAPS unit. Expandable up to 4 HAPS sorting units.

The Cons:Poor sensitivity. Poor sensitivity. Very unreliable, lots of down time, issues with vibration from the hood, major issues with day to day alignment and stability.

l Primary Fluorescence Collection Optic:

u Off-axis parabolic mirror (3x magnification)

u 95% reflection, 300 nm - 1 mm

u Directly coupled into two spatially separated fiber optics

l Secondary Fluorescence Optic (Optional):

u Custom objective coupled into three spatially separated fiber optics

u Transmission > 90%, 400 nm – 850 nm

l Optical Alignment: Automated

l Options:

u AOTF (Acousto-Optical Tunable Filter) with < 3 nm resolution

u 16 channel spectrometer (370 nm to 730 nm)

u Amplitude modulation (either directly or with EOM) from 5 to 40 MHz

u Secondary fluorescence collection optic

u Sort deposition system

u Biohazard containment: Baker SterilGard .

III Advance hood integration

l Dimensions:

l Research Reflection Instrument (W x D x H)

u 45 x 33 x 57 (inches)

u 114 x 84 x 145 (cm)

l DPA (Digital Processing Assembly) (W x D x H)

u 30 x 36 x 32 (inches)

u 76 x 91 x 81 (cm)

l FDA (Fluorescence Detection Assembly) (W x D x H)

u 27. x 16. x 13. (inches)

u 70 x 42 x 34 (cm)

l Electrical requirements: (2) 20 A 120V dedicated lines

l Pressure (5 – 100 psi): House air, nitrogen or optional Jun-Air compressor

l Vacuum (12 inches of Hg): House or optional Jun-Air compressorl HAPS (Highly Automated Parallel Sort) modules: 1 to 4 per instrument

l Analysis Rates: greater than 150,000 events per second for each HAPS

l Sort Rates: greater than 70,000 events per second for each HAPS

l Sort Purity: >99%

l Drop Drive Frequency: Up to 120 kHz

l Sort Directions:

u 32 programmable sort streams

u Separate streams for empty drops and non-sorted cells

u Up to 4 streams collected into tubes

u Tubes supported 0.6 ml, 1.5 ml, 5 ml, 15 ml, 50 ml

u Up to 32 discrete streams for either indexed or proportional sorting

l Fluorescent Detectors: Up to 62 PMTs

l Wide Angle Forward and Back Light Scatter: Up to 8 PMTs

l Narrow Angle Forward Light Scatter: Up to 4 photodiodes

l Streaming ADC inputs (105 MHz Sampling Rate, 14 Bit Resolution): Up to 64 channels

l Listmode Parameters:

u Up to 256

u Pulse features (area, height, width, rise time)

u Sort decisions (cell sort classification, droplet stream classification)

u Time interval between pulses

u Custom programmable pulse features

l Fluorescence Compensation: Full matrix among all features

l Simultaneous Laser Excitation Lines: Up to 7 (355, 405, 488, 532, 561, 592, 640 nm)

l Laser Options: Any vendor, any configuration with industry standard optical table

l Nozzle Sizes: 50 μm to 200 μm

l Measurement Dynamic Range: Greater than 10,000 x

l Measurement Precision: CV of < 1%

l Narrow Forward Scatter Collection Angle: 1 to 5 degrees half-angle

l Optional Wide Forward Scatter Collection Angle: 5 to 41.3 degrees half-angle

l Number of Analysis Regions: Greater than 512

l Number of Separate Sort Gates (Based on Boolean Expressions of Region): Up to 256

User Reviews (3)

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Add Pros & Cons
45
ProScore
Pros
  • 1

    Super fast sorting at 99% accuracy

  • 1

    Very reliable, easy to use software.

  • 1

    Versatile, with 4 way sorting on each HAPS unit. Expandable up to 4 HAPS sorting units.

  • 1

    Cheap and easy to order Raegents and supplies.

  • 1

    11 colors of fluorescence

Cons
  • 2

    poor sensitivity

  • 2

    poor sensitivity

  • 2

    very unreliable, lots of down time, issues with vibration from the hood, major issues with day to day alignment and stability

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