Becton Dickinson LSRFortessa
Released January, 2009
The Pros:Allows precise changes in acquistion rate.
The Cons:No sit flush.
Excitation Optical Platform
The BD LSRFortessa optical layout allows for up to four1 lasers.
355 nm: 20 mW 405 nm: 50 mW 488 nm: 50 mW 640 nm: 40 mW
Power loss at flow cell: <20% of specified laser power
Flow Cell Design
Rectangular quartz cuvette: Internal cross-section, 430 x 180 μm
External quartz cuvette surfaces are anti-reflective coated for optimal transmission of laser light.
Fixed optical assembly with spatially separated laser beams.
The quartz cuvette flow cell is gel- coupled by refractive index-matching optical gel to the fluorescence objective lens (1.2 NA) for optimal collection efficiency.
Forward Scatter Detection
Photodiode detector with a 488/10 bandpass filter
Side Scatter Detector
Photomultiplier tube (PMT) with a 488/10 bandpass filter
Emission Optical Design
Emitted light from the gel-coupled cuvette is delivered by fiber optics to the detector arrays. The BD LSRFortessa uses BD’s patented octagon- and trigon- shaped optical pathways that use signal reflection to maximize signal detection. Please see the separate filter guide for information on dye and filter options.
User Reviews (1)
allows precise changes in acquistion rate
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