5
4 reviews
100

Becton Dickinson FACSCanto


Released January, 2005

Product Shot 1 The Pros:HTS option. Fast acquisition rates (i.e. fast electronics).

Optics

Lasers

Air-cooled: 488-nm solid state, 20-mW laser output

633-nm HeNe, 17-mW laser output

Laser Configuration

Spatially separated beams with 9 x 65- μm elliptical spots

Optical Alignment Procedure

Fixed, no operator alignment required

Flow Cell

180 x 430-μm rectangular quartz flow cell

Collection Optics

Optical-gel coupled 1.2 NA lens

FSC Resolution

1.0 μm

SSC Resolution

0.5 μm

Fluorescence Detector Design

Reflective optics with single transmission filter in front of each PMT

FSC Detector

Photodiode with 488/10 BP

SSC Detector

PMT with 488/10 BP

Fluorescence Detectors

6 PMTs in 4-2 standard configuration

Blue Laser Dyes

FITC, PE, PerCP or PerCP-CyTM5.5, PE-CyTM7 (525, 575, 678 or 695, 785 nm)

Red Laser Dyes

APC, APC-Cy7 (660, 785 nm)

Detector Bands

Blue Laser: 530/30; 585/42; >670; 780/60 nm

Red Laser: 660/20; 780/60 nm

Fluorescence Threshold Sensitivities

FITC <100 MESF; PE <50 MESF

User Reviews (4)

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100
ProScore
Pros
  • 4

    HTS option

  • 3

    Fast acquisition rates (i.e. fast electronics)

Cons

Comments (1)

What's on your mind? See more ProductWiki Talk
William King
William King: #becton_dickinson_facscanto An important consideration of persons considering this instrument should be the noise level generated from the fluidics cart. It can become oppressive after prolonged periods of time. Ideally, the fluidics cart can be hooked up to a house air pressure system (requires quite a high psi, fyi) or connected to a stand alone Jun-Air compressor, the cart compressor can then be turned off and the room will become profoundly quiet. I understand some colleagues have their FACSCanto's fluidics cart inside a cabinet that has doors and this attenuates the noise level as well. Great instrument otherwise! Dec 17, 10
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