3.6
20 reviews
71

Becton Dickinson FACSCanto II


$130,000.00 Released January, 2007

Product Shot 1 The Pros:HTS system for high throughput. Reliable work horse type instrument. Automated QC providing all info about instrument performance (incl. PMT amplification and overall efficiancy, optical background and many others).

The Cons:Service contract is HUGELY expensive, and since it has tons of proprietary stuff no one else except BD can service it. The sales reps were very helpful when we were researching what to buy, but when we ran into problems with HTS they were completely unhelpful. Slow startup routine.

Price: 

approximately $130,000 for the base instrument, more depending on options (please share the price you paid in the comments section below)

Lasers

Air-cooled: 488-nm solid state, 20-mW laser output

633-nm HeNe, 17-mW laser output

Laser Configuration

Spatially separated beams with 9 x 65- μm elliptical spots

Optical Alignment Procedure

Fixed, no operator alignment required

Flow Cell

180 x 430-μm rectangular quartz flow cell

Collection Optics

Optical-gel coupled 1.2 NA lens

FSC Resolution

1.0 μm

SSC Resolution

0.5 μm

Fluorescence Detector Design

Reflective optics with single transmission filter in front of each PMT

FSC Detector

Photodiode with 488/10 BP

SSC Detector

PMT with 488/10 BP

Fluorescence Detectors

6 PMTs in 4-2 standard configuration

Blue Laser Dyes

FITC, PE, PerCP or PerCP-CyTM5.5, PE-CyTM7 (525, 575, 678 or 695, 785 nm)

Red Laser Dyes

APC, APC-Cy7 (660, 785 nm)

Detector Bands

Blue Laser: 530/30; 585/42; >670; 780/60 nm

Red Laser: 660/20; 780/60 nm

User Reviews (22)

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Add Pros & Cons
71
ProScore
Pros
  • 8

    HTS system for high throughput

  • 7

    Reliable work horse type instrument

  • 6

    Automated QC providing all info about instrument performance (incl. PMT amplification and overall efficiancy, optical background and many others)

  • 4

    The most efficient hydrodynamic focusing ever seen. 120 ul sample per min can be run w/o significant changes in both scatter and fluorescence CV.

  • 1

    all service is expensive, not just on this instrument

  • -2

    easy to use

  • -3

    great reliability and sensitivity

Cons
  • 3

    Service contract is HUGELY expensive, and since it has tons of proprietary stuff no one else except BD can service it

  • 2

    The sales reps were very helpful when we were researching what to buy, but when we ran into problems with HTS they were completely unhelpful

  • -1

    Slow startup routine

  • -1

    The HTS system breaks down VERY often

Comments (1)

What's on your mind? See more ProductWiki Talk
porcelli
porcelli: #becton_dickinson_facscanto_ii This is an excellent cytometer; very reliable. However, it has been only moderately successful as a research instrument in our core facility. The reason seems to be that the instrument takes considerable time to start up, which is apparently because of quality check routines that are built into the software. This seems to have been designed with a clinical lab environment in mind; i.e., for an environment in which rigid QC of a formal type is required. In a more flexible research environment, people just find this annoying and it tends to discourage them from working with the instrument! One solution to this would be to just leave the machine on; e.g., have a core person or tech power it up first thing in the AM and then leave it on until the facility closes in the evening. This turns out not to be convenient for us because of personnel limitations, but such an approach would probably solve the major complaint with the instrument (although of course it also burns up laser lifetime unnecessarily). Dec 17, 10
comments (1) like this
  • kbahjat

    kbahjat: One approach that helped our lab was installing a VNC on the workstation and allowing users to connect remotely to initiate the startup routine. It's free and simple to configure, and it lets the users start the instrument without having to physically come to the lab. Jan 7, 11

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