Fixed optical assembly
Air-cooled argon-ion laser, 15 milliwatt, 488 nm; life expectancy >5,000 hours. Optional second laser: nominally 635 nm.
Prismatic expander and achromatic spherical lens providing 22 x 66-μm elliptical beam for argon-ion laser. Nominally 15 x 61-μm elliptical beam for red-diode laser.
Quartz cuvette coupled to emission lens by refractive index matching optical gel for optimum collection efficiency.
Obscuration blade and slit for minimiz- ing unwanted laser radiation at the detector.
Forward scatter detector and filter
High-performance solid state silicon detector with 488-nm bandpass filter for clear signal detection and red-diode (635-nm) laser signal rejection.
Side scatter detector
High-performance photomultiplier using Brewster-angle beam splitter in the emis- sion optical train.
Fluorescence detectors and filters
Up to four high-performance, high dynamic range photomultipliers with bandpass filters: 530 nm (FITC), 585 nm (PE/PI), and >670 nm (PerCP) with base unit, and optional 661 nm (APC) with FL4 option.
Estimated detection limit: 750 molecules of equivalent soluble fluorescein.
Coefficient of variation in FL2-Area of <3%, full peak for propidium iodide- stained chicken erythrocyte nuclei.
Forward and Side Scatter
Forward and side scatter sensitivity
Separation of fixed platelets from noise.
Forward and side scatter resolution
Optimized scatter performance for resolving lymphocytes, monocytes, and granulocytes.
1,024 channels on all parameters.
Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option), providing four log decade range.
Fluorescence compensation networks
Compensation for fluorescence spectral overlap between FL1 and FL2, between FL2 and FL3, and between FL3 and FL4 channels (with FL4 option).
Width and area measurements for discriminating doublets; available for all fluorescence parameters.
Time available correlated to any param- eter for kinetic experiments or other applications.
Front key panel control in three modes: RUN, STNDBY, and PRIME; automatic standby mode for conserving sheath fluid by stopping sheath flow when no sample tube is installed.
Easily accessible 4-L capacity sheath and waste containers housed in a convenient pull-out drawer; level detectors for automatically indicating low levels of sheath or high levels of waste.
Sample flow rates
Three selectable flow rates of 60 μL/min, 35 μL/min, and 12 μL/min; regulated and monitored pressure difference between sheath and sample; particle velocity in flow cell: approximately 6 meters/second.
Internal cross-section: rectangular 430 x 180 μm; antireflection coated external surfaces for maximum transmission of laser light.
Single-cell suspension of 105 to 2 x 107 particles/mL recommended range.
BD FACStationTM Mac Pro computer
2.8-GHz Quad-Core Intel® Xeon® processor
2 GB RAM
Level 2 cache
4 MB per processor
320-GB 7200-rpm Serial ATA, 3Gb/s
On-board Ethernet, built-in AppleTalk® Networking, and Apple® File Sharing
17-inch LCD monitor (optional)
Data file structure
Flow Cytometry Standard (FCS) 2.0/3.0 ASCII results file for data export
Tube-lifter design with multiple sensors that verify rack identification and tube position with optional BD FACSTM Loader.
40 (12 x 75-mm) tubes per rack.
Up to 16 racks per BD FACS Loader.
Sample information, reagent panels, and rack information can be defined for up to 640 tubes (40 tubes x 16 racks) at a time.
Automated control through BDTM Worklist Manager software.
Barcode scanner (optional)
Automates data entry for Codabar, Code 39, Interleaved 2 of 5, Code 2 of 5, and Code 128.
Adjustable high-energy and low-energy mix.
Three modes (all aerosol free): single cell, exclusion, and recovery.
Depending upon sample and sorting conditions, >50%.
System design allows for aerosol-free sterile sorting.
The HTS option allows quick conversion between tubes and plates
384-well plates (flat bottom) 96-well plates (U, V, and flat bottom)
High-throughput (HT) Standard (STD)
Sampling volume (range)
2–10 μL in HT mode 2–200 μL in STD mode
Total aspirated volume
22 μL (HT mode)
Excess aspiration volume
20 μL (STD mode)
Automated daily and monthly cleaning protocols
Dimensions (W x D x H)
Sensor module 91.4 x 61.5 x 67.3 cm (36 x 24.2 x 26.5 in) Height with cover option: 124.5 cm (49 in) Computer 48 x 41 x 58 cm (19 x 16 x 23 in) Printer 48 x 41 x 58 cm (19 x 16 x 23 in)
Sensor module: 109.1 kg (~240 lb) Computer: varies with configuration
US: 120 VAC ±10%, 50/60 Hz ±2 Hz, Current: 20 amp maximum outside US. External transformer needed for 100 VAC ±10%, 50/60 Hz ±2 Hz, and 220/240 VAC ±10%, 50/60 Hz ±2 Hz. BD recommends two dedicated circuits for instruments configured with any of the sorting options listed on the previous page.
BD FACSCalibur system with all options 6,500 BTU/h
10% to 90% relative non-condensing
No excessive dust and smoke
Optics and detectors shielded from room lighting
BD flow cytometers are Class I (1) laser products.
Unless otherwise noted, BD FACSCalibur is for In Vitro Diagnostic Use.