4
11 reviews
79

Becton Dickinson FACSarray


$65,000.00 Released January, 2003

Product Shot 1 The Pros:Very easy to set up, run and analyze full 96 plate analyses, even for lab techs new to flow. A highly reliable workhorse for labs such as ours running antibody clone screens and closely related assays (cell surface antigen binding). Sensitivity for phycoerythrin (PE) seems to be better than most 488nm excitation driven benchtop analyzers (although we have not formally verified this with our Canto II's).

The Cons:Standard software is quite inflexible, and batch analysis/reporting is 'OK' at best in some respects, ie graphs. Tabulated numerical data export is good. Inflexible optics bench; FITC and similar dyes are a no-go in the standard config. The instrument is billed as robotics compatible, but lacks any documentation on how to integrate with plate feeders etc. If indeed possible, BD technical support will be required.

Brief description:
Compact benchtop flow cytometer, with integrated 96-well plate sampler and fixed optical bench

Optics:
Excitation:
2 solid state lasers of 532 and 635nm (10mW each).
There is a custom version not listed in standard catalogs and brochures where a 488nm excitation source replacing the 532nm laser.

Two detectors per laser:
532nm: 585/42BP and >685LP (optimized for PE and PE or PerCP tandems, resp.)
635nm: 661/16 and 780/60 (optimized for APC or Alexa647 and APC-Cy7 tandem, resp.)

Sampler:
robotics compatible plate loader tray, accepting SBS standard format 96 well plates.
No manual tube or other plate format input options available

Fluidics:
Internal sheath and waste containers, with optional ports in back to connect to higher capacity fluids trolley (optional)

Software:
FACSarray control software, an instrument-specific limited flexibility version of BD's DiVA software
Data files can be exported to standard FCS files for third-party software analysis.
There are some provisions for in- and output of experiment metadata in a third party software written specifically for bead array analysis applications.

User Reviews (13)

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79
ProScore
Pros
  • 8

    Very easy to set up, run and analyze full 96 plate analyses, even for lab techs new to flow

  • 6

    A highly reliable workhorse for labs such as ours running antibody clone screens and closely related assays (cell surface antigen binding)

  • 6

    Sensitivity for phycoerythrin (PE) seems to be better than most 488nm excitation driven benchtop analyzers (although we have not formally verified this with our Canto II's)

Cons
  • -3

    Standard software is quite inflexible, and batch analysis/reporting is 'OK' at best in some respects, ie graphs. Tabulated numerical data export is good.

  • -4

    Inflexible optics bench; FITC and similar dyes are a no-go in the standard config.

  • -5

    The instrument is billed as robotics compatible, but lacks any documentation on how to integrate with plate feeders etc. If indeed possible, BD technical support will be required.

Comments (1)

What's on your mind? See more ProductWiki Talk
ghermans
ghermans: #bd_facsarray I bought one just before it was launched on the European market on good faith, many years ago. This device has been ploughing through impossible seeming stacks of microtiter plates since, and is still going as strong as ever. We bought a Canto II to allow for more flexible fluor use as well as the 96/384 high throughput sampler. The latter sits idle most of the time, with the original 'Array still being overbooked most days. The tech love this little cube so much better than the more versatile (hence: bit more complicated) Canto II we ended up buying another 'Array rather than push more plates towards the Canto.
Although marketed almost exclusively as a bead analyzer by BD , we only run cell assays on it and have been highly satisfied with its performance in that area.
There are more options out in the market for this type of instrument than back in the day, and you should carefully evaluate your current and future needs versus the various device configurations (keeping in mind budget restrictions as well). However, for our applications, I'd still happily buy another 'Array. Dec 17, 10
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