4.2
12 reviews
83

Becton Dickinson FACSAria I, II, and III


Released January, 2003

Product Shot 1 The Pros:Reasonably compact size. Lots of automation. Aria II fluidics are great, its ceramic nozzle with glued-in O-ring is the best I have ever seen and used.

The Cons:If any of the automation has issues, no manual fall-back.

Excitation Optics Optical Platform Fixed optical alignment of all Class IIIb lasers upon the cuvette flow cell. The 488-nm and 633-nm lasers come stan- dard. All other laser choices are optional. All are solid state except the 633 nm, which is gas.

Beam height: 9 ±3 μm Beam width: 65 ±7 μm

Power Out of the Laser Head

375 nm: >7 mW, top hat shape 405 nm: >50 mW, elliptical shape 445 nm: >20 mW, elliptical shape 488 nm: >20 mW, elliptical shape 561 nm: >50 mW, elliptical shape 633 nm: >18 mW, elliptical shape

Emission Optics Optical Coupling The quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescent objective lens for optimal collection efficiency.

Numerical aperture: 1.2

Forward Scatter Detector and Filters

Photodiode with 488/10 bandpass filter for the 488-nm laser and 462/52 band- pass filter for the 445-nm laser.

Side Scatter Detector

Photomultipler with a 488/10 bandpass filter for the 488-nm laser and a 445/15 bandpass filter for the 445-nm laser.

Fluorescence Detectors and Filters

Five fixed-fiber apertures for the 488, 633, 561, 445, and 405-nm lasers

The 445-nm laser focuses on the flow cell at the same location as the 488-nm laser. The 375-nm laser is air launched and focuses on the flow cell at the same location as the 405-nm laser. An octagon technology detector array enables user- defined detection configurations.

Wavelengths detected from 488-nm laser without 561-nm laser installed • 530/30-nm FITC • 585/42-nm PE

• 616/23-nm PE-Texas Red® • 695/40-nm PerCP-CyTM5.5 or PI or

675/20-nm PerCP-Cy7 • 760/60-nm PE-CyTM7

Wavelengths detected from 488-nm laser when 561-nm laser installed • 530/30-nm FITC • 695/40-nm PerCP-Cy5.5 or PI or

675/20-nm PerCP

Wavelengths detected from 633-nm laser

• 660/20-nm APC • 780/60-nm APC-Cy7 or APC-H7

Wavelengths detected from 405-nm laser

• 450/50-nm BD HorizonTM V450, Pacific BlueTM, DAPI

• 510/50-nm BD Horizon V500, AmCyan

Wavelengths detected from 561-nm laser

• 582/15-nm PE • 610/20-nm PE-Texas Red®, Living

Colors®, mCherry, propidium iodide • 670/14-nm PE-CyTM5 or 710/50-nm

PE-Cy5.5 • 780/60-nm PE-Cy7 Wavelength detected from 445-nm laser • 510/80-nm, cyan fluorescent protein

(CFP)

Wavelengths detected from 375-nm laser

• 450/20-nm Hoechst Blue, DAPI • 670 LP Hoechst Red, PI

Filters and mirrors are user changeable. Additional detectors up to a total of 18 wavelengths can be added to the arrays.

Steering Optics

488, 633, 561, 445, and 405-nm laser: Fiber optics steer the fixed alignment laser beams onto the expansion prisms to focus them on the cuvette flow cell.

Near UV (375-nm) laser: Air launched and focused on the cuvette flow cell

Sort Performance Drop Drive Frequency Range 1–100,000 Hz

Purity and Yield

At 70 psi and 90 kHz with an average threshold rate of 25,000 events per second, a four-way sort achieved a purity of >98% and a yield >80% of Poisson’s expected yield. Higher threshold rates

up to 70,000 events per second can be achieved without affecting purity; however, yield will decrease based on Poisson statistics.

Viability

Sorts were performed at a variety of sheath pressures using cell lines and human peripheral blood mononuclear cells (PBMCs). All sorts resulted in cells that proved viable and proliferated for several days post-sort.

Sort Collection Devices

Two-way sorting: 12 x 75 mm, and 15 mL

Four-way sorting: 1.5 mL microtube and 12 x 75 mm

Sort Collection Cooling

Water recirculator for refrigeration/ heating (optional)

BD FACSTM Accudrop

• Red diode laser provided for fully automated drop-delay determination

• Automated drop breakoff monitoring

• Automated clog detection and sort tube protection system using Sweet Spot technology

Loading Sample Input Sizes Microtubes, 12 x 75 mm, and 15 mL

Polystyrene or polypropylene tubes can be used.

Sample Input Agitation

Adjustable through the software to keep sample constantly suspended

Temperature Control

Sample input, software-adjustable: 4, 20, 37, and 42°C

Sample output for sort collection: water recirculation unit (optional)

User Reviews (13)

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Add Pros & Cons
83
ProScore
Pros
  • 5

    Reasonably compact size

  • 4

    Lots of automation

  • 4

    Aria II fluidics are great, its ceramic nozzle with glued-in O-ring is the best I have ever seen and used.

  • 2

    great day to day reliability

  • 1

    sensitivity is very good

Cons
  • 1

    If any of the automation has issues, no manual fall-back.

Comments (2)

What's on your mind? See more ProductWiki Talk
porcelli
porcelli: #becton_dickinson_facsaria_i_ii_and_iii No question that the FACSAria II is a solid performer. Size is reasonable for biocontainment within biosafety cabinet if necessary. Optics and electronics are excellent. Fluidics has significant problems with early versions of the instrument, but these seem to have all been cleared up with the more recent releases. I still don't think this sorter can collect cells as fast as the MoFlo, but it's quite fast. Dec 17, 10
comments (2)
  • kbahjat

    kbahjat: Could you elaborate on the biocontainment options? When the Aria I was released they talked about a containment hood that fit around the top part of the instrument, but I'm unaware if that ever materialized. Last time I checked, they were recommending a massive floor to ceiling cabinet. Jan 2, 11

  • porcelli

    porcelli: The standard approach to biocontainment is to place the entire sorter within the Baker BioProtect II biosafety cabinet, which is a rather large essentially floor to ceiling cabinet. This approach has been used in many centers, and it seems to work well. I am not aware of any other currently validated approach for biocontainment of the FACS Aria. Jan 3, 11

William Schott
William Schott: #becton_dickinson_facsaria_i_ii_and_iii We have the FACSAria II SORP. Very big step up versus older FACSVantage/DiVa. We have 5 lasers and like the flexability this gives us. Dec 17, 10
comments (1)
  • Anonymous

    Anonymous: We have a SORP too, and the FSC diode that came with our machine is a much better quality than ships with the standard machine making analysis of bacterial cells easy without a FSC-PMT. Dec 17, 10

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