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Beckman Coulter Gallios
Released January, 2008
The Pros:Like every other Coulter flow instrument, its rock solid alignment and stability. Very fast sample running (> 10,000 events / second). Optics, fluidics, sensitivity.
The Cons:Software. Software. Tardy phone help.
The Gallios Flow Cytometer is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Along with the unprecedented detection capabilities of the instrument, the Gallios includes easy-to-use software and automation to facilitate superior performance of multi-color flow cytometry assays.

Features
The Gallios houses up to three highly reliable, solid-state lasers in standard red and blue, with violet available as an option. Easily interchanged optical filters facilitate detection of a variety of dyes and wavelengths.
An innovative forward-scatter detector provides up to three measurements of cell size and visualization of particles down to 0.404 mm in diameter. A side-scatter detector incorporates an independently focused, high-performance photodiode with electronic attenuation. Six fluorescence detectors provide simultaneous acquisition of up to six fluorescence signals. Up to four fluorescence detectors can be added to the system, enabling concurrent reading of up to 10 colors. Simultaneous measurements of integral, peak and width are available for all parameters, including scatter and fluorescence signals. A selection of up to 62 parameters can be processed per analysis, at acquisition rates of 25,000 events-per-second, with high yield. Three scalable configurations allow for the future addition of more lasers and fluorescence detectors as the applications for complex multi-color flow cytometry expand.
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Available in 3 configurations
6 color 2 Laser (5+1)
8 color 2 Laser (5+3)
10 color 3 Laser (5+3+2)
Innovative laser cartridges
Picomotor driven beam steering optics
Laser control to conserve laser life
Design for laser flexibility
Customer temperature regulation
Plug and play filters allow rapid instrument reconfiguration between experiments
Filters are easily accessible and interchangeable with no tools required
Software features:
Multi-User Administration
Drag & Drop replay of data
Boolean gating and color eventing hierarchy
New log/lin transform for visualization of negative data and compensation optimization
Automatic PDF and .xls creation
Instrument Dashboard
LEDs clearly indicate instrument status from across the lab
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Efficient delivery of light
More power at the flow cell for greater resolution in your applications
Efficient and reliable solid state blue, red, and violet lasers
Free space delivery of laser light
Optimized beam steering optics
Increased power at flow cell
Scatter Parameters
Efficient delivery of light
3 unique setting for relative size measurements
Innovative submicron particle resolution setting for forward scatter
Independent side scatter alignment
Patented electronics design incorporates fast, accurate measurement of signals over a wide range of event rates
Fast pulse recovery
Minimize linearity errors
Sensitivity of dim particles
Minimize dead time
Accuracy at high event rates
PROService Compatible
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User Reviews (27)
Pros & Cons
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8
Like every other Coulter flow instrument, its rock solid alignment and stability
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7
Very fast sample running (> 10,000 events / second)
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5
optics, fluidics, sensitivity
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2
good optics and fluidics.
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1
great stability and sensitivity
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6
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4
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1
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-1
no good plate loader option exists
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Comments (2)
anon:
#beckman_coulter_gallios Excellent optics and fluidics. Carousel run mode only. Very rigid, inflexible software. Not intuitive or user friendly. Expect to waste time troubleshooting this software. Can be guardedly used for acquisition only with a second software package for analysis. If only the software were better this would be a great instrument.
Sep 17, 11
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