3.7
19 reviews
74

Accuri C6 Flow Cytometer


Released January, 2008

Product Shot 1 The Pros:Cheap. Relatively inexpensive. Can change filters.

The Cons:96well mixing is not sufficient. Absolute counts not accurate with very high cell density. 96well sampler is slow, and gets slower if tubing gets old.

Excitation

488nm (rated at 20,000hr life)

640nm (rated at 20,000hr life)

Emission detection           

4 colors, user swappable optical filters

Standard set installed

FL1 533 / 30nm (FITC/GFP)

FL2 585 / 40nm (PE/PI)

FL3 >670nm LP (PE-Cy5, PE-Cy5.5, PerCP-Cy5.5, PE-Cy5, PE-Cy7)

FL4 675 / 25nm (APC)

Minimum particle size: 0.5 µm

Minimum sample size: 50 µl (in a 1.5 mL microfuge tube)

Nominal flow rate - variable: 10 to 100 µl/min ; CFlow Plus only

Fixed Flow Rates & Core Sizes: 

Slow - (Flow = 14uL/min ; Core = 10 um)

Medium - (Flow = 35uL/mim ; Core = 16 um)

Fast - (Flow = 66 uL/min ; Core = 22 um)

Recommended Sheath Fluid: 0.2 um filtered DI water

Florescence sensitivity (MESF) : FITC < 150 , PE < 100

Speed: 10,000 events/second maximum

Absolute counts: Yes; CFlow Plus only

Instrument size

Foot print with fluid tanks   :           11"H x 14.3"W x 16.5"D (27.9 x 36.3 x 41.9 cm)

11"H x 21.5"W x 16.5"D (27.9 x 54.6 x 41.9 cm)

Weight: 30 lbs (13.6 kg)

User Reviews (21)

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Add Pros & Cons
74
ProScore
Pros
  • 9

    Cheap

  • 4

    Relatively inexpensive.

  • 3

    Can change filters

  • 3

    Rather easy to learn, difficult to permanently mess up data when acquired by inexperienced user.

  • 3

    size,

  • 2

    Stable

  • 2

    Absolute counts

  • 2

    ready to set wireless connection

  • 2

    digital (24bit) signal analysis

  • 2

    no vacuum system - ready to set real-time (ex. Ca2+ flux) experiments

  • 2

    small

  • 1

    software - zoom option

  • 1

    set voltage lasers (no extra calibration needed)

Cons
  • 4

    96well mixing is not sufficient

  • 3

    Absolute counts not accurate with very high cell density

  • 3

    96well sampler is slow, and gets slower if tubing gets old

  • 2

    Absolute counts within 96well format is not reliable

  • 1

    BD Accuri C6 system with BD CSampler overheats in periods of 2-9 hrs. The difference in temperature can be quite significant.

  • -1

    The dynamic range of the instruments seems to change with every new version of the software, making it difficult to compare to previous samples.

Comments (2)

What's on your mind? See more ProductWiki Talk
Flowjock457
Flowjock457: #accuri_c6_flow_cytometer I've yet to get a satisfactory response about how the software allegedly deals with the pulsation from the peristaltic pump that drives this instrument. Also, the best PMTs on the market today only garner 4.5 logs of linear data. Where does Accuri get the other 3.5 logs? Sure they may say it is easy to run but part of me thinks they are duping everyone else in the flow world whose machines produce 4.5 logs of usable data. Takes too much control out of the user's hands. Out side of looking like a gumball machine, the C6 doesn't come across as a serious machine, it's lowballed price-wise, I've heard of demos arriving with dead electronics. It just comes across like a bad deal. Dec 17, 10
comments (5) like this
Show all 5 comments
  • Anonymous

    Anonymous: To be honest, this is a weak product and software. I am running samples on it now and have to unclog at every sample, very annoying. Sep 15, 12

  • Anonymous

    Anonymous: Had one for 2 years now. Love it. Little workhorse that is great for everyday/quick use. The extra logs are from larger acquisition chips I believe and eliminate need for PMT voltage adjustments making compensations easier. Mine has never clogged. Jan 7, 13

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